Royal College of Surgeons in Ireland
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Versatility of unsaturated polyesters from electrospun macrolactones: RGD immobilization to increase cell attachment

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journal contribution
posted on 2021-10-22, 15:02 authored by Fernando Cabral Sales de Oliveira, Ronaldo Jose Farias Correa Do Amaral, Luiza Erthal Cardoso dos Santos, Cian Cummins, Michael M. Morris, Cathal KearneyCathal Kearney, Andreas HeiseAndreas Heise
Poly(globalide) (PGl), an aliphatic polyester derived from unsaturated macrocylic lactone, can be cross-linked during electrospinning and drug-loaded for regenerative medicine applications. However, it lacks intrinsic recognition sites for cell adhesion and proliferation. In order to improve their cell adhesiveness, and therefore their therapeutic potential, we aimed to functionalize electrospun PGl fibers with RGD sequence generating a biomimetic scaffold. First, an amine compound was attached to the surface double bonds of the PGl fibers. Subsequently, the amino groups were coupled with RGD sequences. X-ray photoelectron spectroscopy (XPS) analysis confirmed the functionalization. The obtained fibers were more hydrophilic, as observed by contact angle analysis, and presented smaller Young's modulus, although similar tensile strength compared with non-functionalized cross-linked fibers. In addition, the functionalization process did not significantly alter fibers morphology, as observed by scanning electron microscopy (SEM). Finally, in vitro analysis evidenced the increase in human mesenchymal stromal cells (hMSC) adhesion (9.88 times higher DNA content after 1 day of culture) and proliferation (3.57 times higher DNA content after 8 days of culture) compared with non-functionalized non-cross-linked fibers. This is the first report demonstrating the functionalization of PGl fibers with RGD sequence, improving PGl therapeutic potential and further corroborating the use of this highly versatile material toward regenerative medicine applications.


Governo Brasil-CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior) Grand Number No. 13695/2013-04

Science Foundation Ireland (SFI), European Regional Development Fund (Grant Number 13/RC/2073)

European Union's Horizon 2020 research and innovation program under the Marie Skłodowska-Curie Grant Agreement No. 713690



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Published Citation

de Oliveira FCS, et al. Versatility of unsaturated polyesters from electrospun macrolactones: RGD immobilization to increase cell attachment. J Biomed Mater Res A. 2021

Publication Date

28 July 2021

PubMed ID



  • Chemistry
  • Anatomy and Regenerative Medicine
  • Tissue Engineering Research Group (TERG)
  • CURAM Centre for Research in Medical Devices
  • Amber (Advanced Material & Bioengineering Research) Centre

Research Area

  • Biomaterials and Regenerative Medicine
  • Chemistry and Pharmaceutical Sciences




  • Published Version (Version of Record)