Zerumbone liquid crystalline nanoparticles protect against oxidative stress, inflammation and senescence induced by cigarette smoke extract in vitro
The purpose of this study was to evaluate the potential of zerumbone-loaded liquid crystalline nanoparticles (ZER-LCNs) in the protection of broncho-epithelial cells and alveolar macrophages against oxidative stress, inflammation and senescence induced by cigarette smoke extract in vitro. The effect of the treatment of ZER-LCNs on in vitro cell models of cigarette smoke extract (CSE)-treated mouse RAW264.7 and human BCi-NS1.1 basal epithelial cell lines was evaluated for their anti-inflammatory, antioxidant and anti-senescence activities using colorimetric and fluorescence-based assays, fluorescence imaging, RT-qPCR and proteome profiler kit. The ZER-LCNs successfully reduced the expression of pro-inflammatory markers including Il-6, Il-1β and Tnf-α, as well as the production of nitric oxide in RAW 264.7 cells. Additionally, ZER-LCNs successfully inhibited oxidative stress through reduction of reactive oxygen species (ROS) levels and regulation of genes, namely GPX2 and GCLC in BCi-NS1.1 cells. Anti-senescence activity of ZER-LCNs was also observed in BCi-NS1.1 cells, with significant reductions in the expression of SIRT1, CDKN1A and CDKN2A. This study demonstrates strong in vitro anti-inflammatory, antioxidative and anti-senescence activities of ZER-LCNs paving the path for this formulation to be translated into a promising therapeutic agent for chronic respiratory inflammatory conditions including COPD and asthma.
Funding
Open Access funding enabled and organized by CAUL and its Member Institutions
University of Technology Sydney, Australia: Global Strategic Partnerships Seed Funding Scheme
International Medical University, Malaysia
National Health and Medical Research Council (NHMRC) of Australia (1059238, 1175134, 2010287)
Prevent Cancer Foundation (PCF), Alexandria, VA, USA
International Association for the Study of Lung Cancer (IASLC), Denver, CO, USA.
Rebecca L Cooper Medical Research Foundation
Maridulu Budyari Gumal Sydney Partnership for Health, Education, Research and Enterprise (SPHERE) RSEOH CAG Seed grant fellowship and extension grant
Faculty of Health MCR/ECR Mentorship Support Grant.
History
Comments
The original article is available at https://link.springer.com/Published Citation
Paudel KR. et al. Zerumbone liquid crystalline nanoparticles protect against oxidative stress, inflammation and senescence induced by cigarette smoke extract in vitro. Naunyn Schmiedebergs Arch Pharmacol. 2024;397(4):2465-2483.Publication Date
18 October 2023External DOI
PubMed ID
37851060Department/Unit
- School of Pharmacy and Biomolecular Sciences
Publisher
Springer VerlagVersion
- Published Version (Version of Record)