miR-222 isoforms are differentially regulated by type-I interferon.
Endogenous microRNAs (miRNAs) often exist as multiple isoforms (known as "isomiRs") with predominant variation around their 3'-end. Increasing evidence suggests that different isomiRs of the same family can have diverse functional roles, as recently demonstrated with the example of miR-222-3p 3'-end variants. While isomiR levels from a same miRNA family can vary between tissues and cell types, change of templated isomiR stoichiometry to stimulation has not been reported to date. Relying on small RNA-sequencing analyses, we demonstrate here that miR-222-3p 3'-end variants >23 nt are specifically decreased upon interferon (IFN) β stimulation of human fibroblasts, while shorter isoforms are spared. This length-dependent dynamic regulation of long miR-222-3p 3'-isoforms and >40 other miRNA families was confirmed in human monocyte-derived dendritic cells following infection with
Australian National Health and Medical Research Council (1062683, 1069128 and fellowship 1026191); the Australian Research Council (140100594 Future Fellowship); the Canadian Fonds de recherche du Québec – Santé (35071 FRSQ Fellowship). European Union's Seventh Framework Programme (FP/2007–2013)/ERC grant agreement no. 281297.
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Published CitationNejad C, Pillman KA, Siddle KJ, Pepin G. Anko ML, McCoy CE, Beilharz TH, Quintana-Murci L, Goodall GJ, Bracken CP, Gantier MP. miR-222 isoforms are differentially regulated by type-I interferon. RNA. 2018;24(3):332-341
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