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miR-CATCH: microRNA capture affinity technology.

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journal contribution
posted on 2022-03-30, 14:09 authored by Sebastian Vencken, Tidi HassanTidi Hassan, Noel G McElvaneyNoel G McElvaney, Stephen GJ Smith, Catherine M. Greene, Catherine GreeneCatherine Greene

Several experimental methods exist to explore the microRNA (miRNA) regulome. These methods almost exclusively focus on multiple targets bound to a single, or perhaps a few miRNAs of interest. Here, we describe a microRNA capture affinity technology (miR-CATCH) which uses an affinity capture oligonucleotide to co-purify a single target messenger RNA (mRNA) together with all its endogenously bound miRNAs. This bench-top method is similar to RNA immunoprecipitation (RIP) and provides an experimental alternative to computational miRNA target prediction.


Science Foundation Ireland



The final publication is available at Springer via 10.1007/978-1-4939-1538-5_23

Published Citation

Vencken S, Hassan T, McElvaney NG, Smith SG, Greene CM. miR-CATCH: microRNA capture affinity technology. Methods Molecular Biology. 2015;1218:365-73

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  • Beaumont Hospital
  • Medicine