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miR-CATCH: microRNA capture affinity technology.

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journal contribution
posted on 30.03.2022, 14:09 authored by Sebastian Vencken, Tidi HassanTidi Hassan, Noel G McElvaneyNoel G McElvaney, Stephen GJ Smith, Catherine M. Greene, Catherine GreeneCatherine Greene

Several experimental methods exist to explore the microRNA (miRNA) regulome. These methods almost exclusively focus on multiple targets bound to a single, or perhaps a few miRNAs of interest. Here, we describe a microRNA capture affinity technology (miR-CATCH) which uses an affinity capture oligonucleotide to co-purify a single target messenger RNA (mRNA) together with all its endogenously bound miRNAs. This bench-top method is similar to RNA immunoprecipitation (RIP) and provides an experimental alternative to computational miRNA target prediction.

Funding

Science Foundation Ireland

History

Comments

The final publication is available at Springer via 10.1007/978-1-4939-1538-5_23

Published Citation

Vencken S, Hassan T, McElvaney NG, Smith SG, Greene CM. miR-CATCH: microRNA capture affinity technology. Methods Molecular Biology. 2015;1218:365-73

Publication Date

2015-01-01

PubMed ID

25319664

Department/Unit

  • Beaumont Hospital
  • Medicine