miR-CATCH: microRNA capture affinity technology..pdf (660.61 kB)
miR-CATCH: microRNA capture affinity technology.
Version 2 2022-03-30, 14:09
Version 1 2019-11-22, 16:34
journal contribution
posted on 2019-11-22, 16:34 authored by Sebastian Vencken, Tidi Hassan, Noel G. McElvaney, Stephen GJ Smith, Catherine M. GreeneSeveral experimental methods exist to explore the microRNA (miRNA) regulome. These methods almost exclusively focus on multiple targets bound to a single, or perhaps a few miRNAs of interest. Here, we describe a microRNA capture affinity technology (miR-CATCH) which uses an affinity capture oligonucleotide to co-purify a single target messenger RNA (mRNA) together with all its endogenously bound miRNAs. This bench-top method is similar to RNA immunoprecipitation (RIP) and provides an experimental alternative to computational miRNA target prediction.