Three bis(anilino)-substituted NIR-AZA fluorophores have been designed, synthesized and tested to bridge the availability gap of molecular fluorophores for live-cell microscopy imaging in the 800-850 nm spectral range. The concise synthetic route allows for the later stage introduction of three tailored peripheral substituents which guides the sub-cellular localization and imaging. Live-cell fluorescence imaging of lipid droplets, plasma membrane and cytosolic vacuoles was successfully achieved. Photophysical and internal charge transfer (ICT) properties of each fluorophore were examined through solvent studies and analyte responses.
Funding
Irish Government Department of Business, Enterprise and Innovation's Disruptive Technology Innovation Fund
Synthesis and Solid State Pharmaceutical Centre (SSPC)
Science Foundation Ireland, grant number 12/RC/2275_P2
RCSI Apjohn Fellowship
Science Foundation Ireland (18/RI/5723)
History
Comments
The original article is available at https://pubs.rsc.org/
Published Citation
Caulfield C, Wu D, Garre M, O'Shea DF. Substituent directed cellular imaging in the 800-850 nm range with BF2-azadipyrromethene fluorophores. RSC Adv. 2023;13(22):14963-14973.